FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_10_bzip11_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_10_bzip11_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15779658
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT11700.014.3616431
CGGGCAT18450.014.0405491
CCCTACA21500.013.99723210
CGGGGGG15800.012.1858091
TCCCTAC27700.011.7484689
CGGGGAC17900.011.7340251
CGGCAAT33750.011.6169731
GGGGCAT17250.010.9585581
CGCGGGG15200.010.5940741
GGATAAC99900.09.9517991
GGGGTAT13450.09.6300421
CTACACG31750.09.36849412
CGGGAAT40300.09.2076661
TCGGGCG17150.09.1818017
CGGGCCC4201.00902216E-49.168371
GGGGAGC25350.08.9760271
CGCGTAT24750.08.9107471
CGGGGGT16900.08.9069811
GGGGAAT46650.08.8548071
CGGGCGC12650.08.85231415
Produced by FastQC (version 0.11.2)