FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_09_bzip11_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_09_bzip11_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17016601
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT14100.014.6483251
CCCTACA23000.014.14956210
CGGGGGG18550.013.39891051
CGGGCAT19650.013.3614551
GGATAAC122150.011.5209321
TCCCTAC31200.011.4400279
GGGGTAT15250.011.2481561
CGGGCAC16700.011.110011
CGGGCCC4752.8903742E-811.0548461
ACATGGC135800.010.1033066
CTACACG33000.09.86201812
GGCCATC129150.09.75429610
CATGGCC135800.09.7404697
CGGGGGT15100.09.737051
CGGGGAG26300.09.7167751
GGCCACG131300.09.572618565
ATGGCCA135650.09.519048
CATGCGC131350.09.51074428
CGGCAAT32800.09.3921261
ATGCGCT134000.09.34877329
Produced by FastQC (version 0.11.2)