FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_08_bzip1_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_08_bzip1_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15137022
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG297850.1967692192030903No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC271600.17942763114171334No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG163770.10819169054520764No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC158820.10492156251077656No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG158240.1045383959936109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55900.021.355211
CTAGCAG63700.019.227468
ACTCTAG78500.017.2544335
CTGACTC75300.016.31422
GACTCTA84200.016.1278364
GTGCGCA123450.015.62686968
TGCGCAT129650.015.01445169
CCCTACA19100.014.65721410
GGTGCGC133000.014.61047367
TCTATCG83850.014.52368116
TAGCAGA85700.014.454789
GCGCGCC67400.014.38845362
CTATCGA85650.014.259171517
CTCTAGC97750.013.9996746
TGACTCT98150.013.621513
AGGTGCG142750.013.61197766
CGCGGGG13000.013.4644271
TCTAGCA153350.013.3952417
AGCGCGC73400.013.25976561
CGGGCAT20400.013.213621
Produced by FastQC (version 0.11.2)