FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_07_bzip1_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_07_bzip1_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147505
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG335900.18509431461790476No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC299350.16495380494453646No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG185440.10218484579560662No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG184090.10144094188154239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT63550.020.3264181
CTAGCAG71100.017.8168548
GTGCGCA148600.016.51629668
GCGCGCC70000.015.80511862
TGCGCAT156450.015.73229369
ACTCTAG93800.015.6711025
GGTGCGC157300.015.66992867
CTGACTC84950.015.2439462
GACTCTA98900.015.1815624
AGGTGCG166650.014.81135866
AGCGCGC75550.014.73649361
CGAGCGC75850.014.53949459
TAGCAGA98750.013.6076339
GCGCCGA85550.013.13663564
CTATCGA97050.012.76455717
CCCTACA23650.012.72518610
ACGAGCG87450.012.65093858
CGGGCAT20900.012.5621791
TGACTCT114050.012.4898423
CTCTAGC118400.012.4740956
Produced by FastQC (version 0.11.2)