FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_06_bzip1_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_06_bzip1_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37301061
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG741320.19873965515350892No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC657660.17631133870427976No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530450.14220775114144876No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG427680.11465625602446002No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG400310.10731866313400576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT129100.021.6392611
CTAGCAG144000.020.1940678
ACTCTAG189950.017.246715
GTGCGCA311550.016.6323468
GACTCTA204300.016.206624
CTGACTC181600.015.88130952
TGCGCAT326800.015.834578569
GGTGCGC329750.015.70393867
CGGGCAT38550.015.3471621
AGGTGCG351800.014.72879466
GCGCGCC139950.014.63471562
TAGCAGA207650.014.4253369
CCGGGGT29800.014.0971321
AGCGCGC145300.014.02329161
TGACTCT227300.013.5966573
CTATCGA206450.013.35672917
TCTAGCA378300.013.264727
CGAGCGC156050.013.16953359
CTCTAGC249650.013.1083146
TCTATCG210700.013.00456716
Produced by FastQC (version 0.11.2)