FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_05_bzip1_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_05_bzip1_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18645762
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC295750.15861513195330929No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG293030.15715635542274967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT65800.022.345361
CTAGCAG75700.020.2472618
CTGACTC89900.016.8184762
ACTCTAG99350.016.486745
GACTCTA103050.016.4722024
GTGCGCA137800.015.54936868
CTATCGA97950.014.89847417
TAGCAGA106750.014.6529999
GGTGCGC147950.014.55384367
TGCGCAT147700.014.50681869
TCTATCG100950.014.4904716
TGACTCT112800.014.2417293
AGGTGCG159150.013.55094166
CTCTAGC130200.013.1716296
AGCGCGC76050.013.12007761
GCGCGCC75300.013.11144962
TCTAGCA191100.012.6350017
GATCTAT118500.012.55113714
CCGGGGT14850.012.4943411
ATCTATC120250.012.28117415
Produced by FastQC (version 0.11.2)