FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_04_ev_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_04_ev_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565179
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264050.1594006318917532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210370.12699530744581752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC6000.015.1707771
CCCTACA24050.013.67800810
CGGGCAT18400.013.1285581
TCCCTAC28250.012.7591279
CCGGGGT13300.012.1085441
CGCGGGG15500.011.2933841
CGGGGGG20350.011.1823921
CGGGGGT15150.011.0921151
CGGGGAC18000.010.5028471
GGATAAC113600.09.9851011
CTACACG33950.09.89576512
GGGGCAT14050.09.7179361
GGGGCAC8503.6379788E-129.4731561
GGCCATC109700.09.31507310
CGGGCCT12100.09.2587081
CGGACAT26100.09.2553821
CCTACAC38400.09.20463911
GGGCAAT35900.09.166831
ACATGGC124550.09.1611126
CGGGGAG24250.09.0952481
Produced by FastQC (version 0.11.2)