FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_03_ev_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_03_ev_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6669814
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1014771.5214367297198992No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT987281.4802211875773448No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC101850.15270290895668157TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74140.11115752253361187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17700.045.67933744
GTATGCC18150.044.35394745
CTCGTAT20300.039.13411742
TATGCCG21650.037.83023546
TAGGCAT21400.037.61089735
TCGTATG21750.036.8491943
TTAGGCA23000.035.4509434
CACTTAG24100.033.39723231
CAGTCAC27100.029.82925227
AGTCACT28000.029.24539428
ATGCCGT29200.028.04878847
GCACACG29750.027.99643111
ACGTCTG29750.027.99643115
CACACGT29950.027.92632512
TCACTTA29600.027.7827930
GGCATCT29100.027.65908237
CCAGTCA30400.027.16697926
GTCACTT30500.027.07770529
GCCGTCT30150.026.81833549
CACGTCT31900.025.99982314
Produced by FastQC (version 0.11.2)