FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_02_ev_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_02_ev_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21660879
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG219690.10142247689948315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT25150.014.6160241
CCGGGGT16250.014.2189821
GGATAAC157700.011.9878151
CGCGGGG18650.011.8260481
CGGGGGG26950.011.691271
GCGGGCC9600.010.9402681
GGGGCAC14300.010.5271391
GGGGTAT18850.010.400511
CGGGGAC24300.010.3729941
GGGGCAT21650.010.1873351
CGGCAAT41550.010.1108621
GGCCATC164600.09.92865810
ACATGGC178150.09.9020116
CGGGGAG34650.09.8004591
CATGGCC176300.09.6863917
TCGGGCG19550.09.6658817
ATGGCCA174700.09.4345348
GATAACA198250.09.3571882
CCCTACA25600.09.29549310
TGGCCAT177600.09.2805219
Produced by FastQC (version 0.11.2)