FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_01_ev_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_01_ev_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19423399
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG196230.10102763167249974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG20850.013.7674971
CGGGCCC7550.012.9824981
CGGGGGT18750.011.5754421
CGGGCAT22150.011.3790491
GGATAAC142300.011.2915871
GGGGCAT20800.011.1077941
CCGGGGT15300.010.7535931
CGGGGAG32000.010.3925191
GGGGTAT14900.010.3374541
GCGGGAT38400.09.8455451
CGGGGAC24450.09.7359261
GGGGAAT54150.09.6970581
GCGGGCT20500.09.5627181
GCGGGGG25450.09.4909241
GGGGACT33550.09.2863431
CGCGGGG17850.09.2173661
ACATGGC167500.09.1729886
GGCCATC151900.09.0077510
CGGCAAT39100.08.9530381
TAACGTA58300.08.9450144
Produced by FastQC (version 0.11.2)