FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494828046
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13097490.26468770527206537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG17745300.062.8049352
AAGCAGT17811500.062.7925261
GCAGTGG20432800.054.469313
AGTGGTA22198400.050.2072535
CAGTGGT24092550.046.2308044
GTGGTAT25403650.043.8782276
GGTATCA26083300.042.6508648
TGGTATC26681950.041.731297
GTATCAA26655100.041.6212279
ACGCAGA25375600.025.02163514-15
CAACGCA25388050.025.00212-13
ATCAACG26098500.024.41857710-11
AGTACAT26010600.024.35175120-21
GCAGAGT26202650.024.3094116-17
AGAGTAC26551950.024.04032318-19
TACATGG26328000.023.93965122-23
CATGGGG16493900.023.518924-25
GTACATG25047400.022.10791222-23
CGCAGAG26019200.021.48456216-17
TATCAAC26198250.021.27880110-11
Produced by FastQC (version 0.11.5)