FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n02_Ctr_9h_14h_cutOct25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n02_Ctr_9h_14h_cutOct25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTTAGGCGTGATGCACCTCCTGGACAGAAGGTTGGTCTTATTGCT11.2345679012345678No Hit
GTCACGGTGACGAAGACGATGAGCACCACCACTGATTCCGGCCTCTCCCT11.2345679012345678No Hit
CATATGTTAGGCATAAGGGAAGGAAGTTTGAGAAAGCTAGAGGAAAGCGA11.2345679012345678No Hit
CAACTGGTCATGGTTTCCAATATGATTACTCCTTGTTGGATATCAAGAGG11.2345679012345678No Hit
AACAGGAGGACCGTGAACCGTGCTTATGGTGGGGTTTTGTCTGGTTCTGC11.2345679012345678No Hit
TCAAAGCCTTTCTGACTCGGCCTGGCCCGGTCGCCCTACGCTACTGGCGC11.2345679012345678No Hit
GAGTCCCTTTCGATTGTTTTCCCATGATAGTGATGAAAAAAAACTGTTCA11.2345679012345678No Hit
GTTGAAACTTCCGGGAAAAAACTTCGAATTGGGAGGGCGATCCTCCCGGT11.2345679012345678No Hit
AGAAGTTGATGAGATGATTAAGGAAGCTGATGTTGATGGTGATGGTCAGA11.2345679012345678No Hit
AATTTGAAGAAGAGGACACCTAAGCCGAAAGCTGCCTAGTATCAATATTA11.2345679012345678No Hit
CGAGGACTTCATCAGCCATTTTCTTCCCTCCTTCCAACTGAAGAAGGAGA11.2345679012345678No Hit
CTTGGACTTGGCAAGTGACTAATCATCAACTCGCAACCTTATGAGTGCTA11.2345679012345678No Hit
GCATTGAAGCCGAGTGTCCAAAACTGATTGCTTGGGGTAAAAGGTGGGTG11.2345679012345678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGC11.2345679012345678No Hit
ACAAGAAGACCAAGAAGCTCCCTCCCGTCTGGAAGTACGAATCCACTACC11.2345679012345678No Hit
AGGTAGAGAAGTTCAAGAAGTCCCTTGGGATCGCCGTTTACAAGCTTCAT11.2345679012345678No Hit
GTGGTATCAACGCAGAGTACATGGGTATCGTTTCTGGTCCTACATTTTTG11.2345679012345678No Hit
CGCGGTGCGCGTCAGGGGGTGTGATTGCGGGGGTTCGGTGGGCAGAGGAG11.2345679012345678No Hit
GGTGGATGTGGTGTGGTGGTGGGGGTGGGTGCGTGGAGTGGGTGGGTTGC11.2345679012345678No Hit
CGAAAGCAGCACAAGAAGGACGCAGCACAAGAGGCTGTGAAGAGAAGGAG11.2345679012345678No Hit
GTCGTGGAAGGCTTAGACGTGGTAAAGGCCATCGAGAAGGTTGGATCATC11.2345679012345678No Hit
CCCAAGTTCAAGCTCATCACCCCTTCCATTCTCTCTGACCGTATGAGGAT11.2345679012345678No Hit
GCAAAAGCAGTGGTGGTATTGCCCGTCACATCCTCCAACAGCTGGAGACA11.2345679012345678No Hit
GATCAGCCTCTTTAGTTTGATTAGTGTTCTGAATGTTTCTAATTATGCAG11.2345679012345678No Hit
GTAATAAAAAAAAGAGCTTGGCATTTGTTTTGTATTCTTGTATTGTCTGC11.2345679012345678No Hit
GCTAAAGCCAGAGGAGAGACTATCAGCACCAAGAGACAGCCTAAAGGTCC11.2345679012345678No Hit
GGTTGCTAACAGTTCCAAGTGCCAAGACCCTTGGCGAATGGGCTGGTCTC11.2345679012345678No Hit
TTGTAGGAAAGAGAACCAGATACCGTCTTGATGGCACCAAGATCATGAAG11.2345679012345678No Hit
TTTCATTTTTTTTCTTCCAAAGTTTGTTTGTTGCTGTATCATTGTAGATG11.2345679012345678No Hit
ATCCGCTCCCAGCTCGCCAGTGCCAAGTGAAGAAAAGCTTGATTTGAACA11.2345679012345678No Hit
AAGAAGGAGATGTATTTCCCAATAAGGAAGTACGCTATCAAAGTTTAAGT11.2345679012345678No Hit
GAGAAGAATCAAAAGCAGCAAGCAACCAGTTTCTTCTTCCTTATGTTTAT11.2345679012345678No Hit
AAGAAGATGCAGAAGCTCAGGTGCAGAACTGAAAATAGCTTTTAACTATT11.2345679012345678No Hit
TTTGTCTTCTCAGGATGTCCCAACTCCTTACGCCGGTACATTGGAAGAAT11.2345679012345678No Hit
GGGGGGAGCATATGCAAAGAACAGTTTTGGTAATATGTAAGCTCATTTAT11.2345679012345678No Hit
GGTGTCTGTCATGTCTTCCATGGGAGAGGAGCAGATCTGTGCCGTCAAGG11.2345679012345678No Hit
TTTCAGAGAGGGTACCGAAGCCAAGCCAAGGAACAAGGGAGTTGCTTCAT11.2345679012345678No Hit
GTCTGGAGGCTCCGGCTTCGCGGCAGTGGCGCGAGAACACGCTATGGGGG11.2345679012345678No Hit
CTTGATCTTAGGGCAGTTTGCCATTAGAAGTGATAATAATTGAGTTACGT11.2345679012345678No Hit
GGTGTGGGTTTTTGTTGTTGATGCTGTTGTAGTGGGGTTTGTTTGCTTGG11.2345679012345678No Hit
GGAACTGGAATGAGAAGAGCGATGAATGAAGGTCGAGATCACAAAACCAT11.2345679012345678No Hit
AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCT11.2345679012345678No Hit
GAAAAGGCAAAGATCTCTCACTTGATTGTCTTTGAAAGAGAAGATCGTTC11.2345679012345678No Hit
CTAAGAGGAAGTTGGGTACTCACAAGAGAGCTAAGAGGAAGAGAGAGGAG11.2345679012345678No Hit
CTTCATAACACCGATTCAGATTGTTGTAGCATTGGCTGGATTCTTCACGA11.2345679012345678No Hit
AAATTGGCAATAGGAAAAAGTTCACATCTCTTTGCTTTTAATTATGGATA11.2345679012345678No Hit
AGCTGCTCCAGCACCTGCTGCTACACCTGCTCCAACTGCAGCTGTACCGA11.2345679012345678No Hit
CATGGGCATCGATTCCACCCGCCTAGGTTGGATGGGCGTGCTTCGTCGGA11.2345679012345678No Hit
CTTGAAGCTCTGGGATACTCTCCGGAGATGCAATTCCAGTTCTGATCTGA11.2345679012345678No Hit
TTAAAGCCGGTACCAAGGGTAGACAGTTCGGGTGAGGTTTGCGGTGTAAC11.2345679012345678No Hit
CACCCAAGAGATGTCAACTGCAGGAAGAAGAAGTGTGGTCACAGCAACCA11.2345679012345678No Hit
AGCTTATCGTGCAGAAACGTAGGGCTAACCTTAACGACTTTGATAGGTTC11.2345679012345678No Hit
TGATAAGATTGCGACTTTGGTCAAAGCGGCTGGTGTTGAGATTGAGTCTT11.2345679012345678No Hit
ACAACAGGAAACAAGAGAGGAGGTGGTGGTGGTTCTAATGCTGATGCAAG11.2345679012345678No Hit
AGGGGGATCACCGAAGAGGCCAGGGGCAAGGCTTCCGATAAGAGTCGGTT11.2345679012345678No Hit
GGACCCTCCCAGAAGTTCTCCGCTATCGTCGAAGAGGTGACGGTCCTTCC11.2345679012345678No Hit
GTGGAGGATGGTAATTCCTTTAATTAGGTTTGGGATTACCAATGAATGTT11.2345679012345678No Hit
GGGATGCTCTTTCAGGAAGAAGTGAGATTGAGATCTCACGAATCATTCAT11.2345679012345678No Hit
TCATGACTCCCCGGTTAATTTGATTTCTTCTATCATGAATCATCCTTTAT11.2345679012345678No Hit
ATCTGAAGGAAGTAGCGACTCTTGATGCTCTTAAGAGTAAGGATAAGAAG11.2345679012345678No Hit
TGGGGGGGGTGTGGGGGGTTGGTTGGGGGTGGGTGTGTGTGGGGTGGGTG11.2345679012345678No Hit
CTCGCATTTTGGAGGTAGATGGGAAGCTGAATCCAACAAAGCAAGCTACT11.2345679012345678No Hit
AAAGGGTAAAGGTATTAAGTTGACCATCATTGAGGAAGCCAGGAAGAGAC11.2345679012345678No Hit
TTCATGTAAAACGTCTCTGATTGACTCTTTTTTGGGGTGGAATCGATGTT11.2345679012345678No Hit
TGGAAACGTTGTTGGCCGTTTCTCACCGGATTCGCCGTTACCGGCGTTCT11.2345679012345678No Hit
GAACAACAGTATCTCTCTCCGTCGTTACCGGTGATCAGTTTTATGTTTTG11.2345679012345678No Hit
GGGAGATATTCCCGGAGTTAGGTTCAAGGTCGTGAAGGTTTCTGGTGTTT11.2345679012345678No Hit
GACACCGTCCACAAACGGAGCATCTGACGTCGACCAGAACCGACACGTGC11.2345679012345678No Hit
AAATTTGCTCCTCCCAAAACCCCTGGAGTTGATGATATTACTGGAGAGCC11.2345679012345678No Hit
GTGATCGACACATTTGAGAAAGTGCCAGAGGAAGAGGAAGGAGCAGGCGA11.2345679012345678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCTATCTCACTCTAAAAT11.2345679012345678No Hit
AAAGTTTCGAGTTTTGAGTTTTCTTCTAGTATGAGGTTTGGATTTGGATC11.2345679012345678No Hit
CTGAGGTCGGAGAGTCTCTACCGCTGAACCATTCTACAACAGTCTCTGCT11.2345679012345678No Hit
GGAAGTGGCGACTCTTGATGCTCTTCAGAGTAAGGATAAGAAGGTTGCTG11.2345679012345678No Hit
ATGGAAATAACGTTGATTTGGGAACAGCTTGCGGAAAGTACTTCCGTGTT11.2345679012345678No Hit
AGTTCTGTGGCAAGTACGGAGTGAAGCGAAAGGCTGTTGGTATCTGGGGT11.2345679012345678No Hit
ACTTGATTCACAGAAGCACATTGACTTTGCCCTCACCAGTCCCTTCGGTG11.2345679012345678No Hit
CGGTCAGACACACCTGATGTTTCAAGTCAACTTTGAGCTTATGTTCTTAA11.2345679012345678No Hit
TAAGTGGAATCTTTTGTGACTGTGTTACTTGCATTTATTTTTATGTCGTC11.2345679012345678No Hit
TCATGACTATTGTTGCAAACCCAAGACAGTTCAAGATTCCAGACTGGTTT11.2345679012345678No Hit
GGAGGTGGTGCTCCGGTTGCAGCTGCTGCTCCAGCTGCTGGCGGTGGTGC11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATT50.085.09
GAGTCCC50.085.01
GTCCCTT50.085.03
CCTTTCG50.085.06
CCCTTTC50.085.05
AGTCCCT50.085.02
TCCCTTT50.085.04
TTTCGAT50.085.08
CTTTCGA50.085.07
AAAAAAA100.042.536-37
Produced by FastQC (version 0.11.5)