FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n02_9hCut_9h14h_Oct25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n02_9hCut_9h14h_Oct25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGAGTTGCTGATTAGGCTCCTCATGCTGACACTGCCCTCGCCTCTTT11.3888888888888888No Hit
GGCACATATACGAGCATAGCGTGCACAAGCGAGTTATATCTAATTTAAGC11.3888888888888888No Hit
GTTCGTCGTACCGCTGTCAAGGTTTCCAGTACAAGAGGTGGAAGTTGGGG11.3888888888888888No Hit
CCAAGCCCTTACCAGCGAGCCCGGCTTAGCCCAGCGTACAAGAGGGATGA11.3888888888888888No Hit
ACTGGAGTTTTTAGGTGTCACTTGGAATCAAAACTCTAGTTTGAGATATT11.3888888888888888No Hit
CTGGCTGGTGGTGTCGCTATTAGCGGGGTCAGCTGATCGCAGGTGTGTGT11.3888888888888888No Hit
CTGAAATGCACGAAGAAGAATAGTTGCCTTAGAAACAAAATGATGCAACA11.3888888888888888No Hit
CACTGTCCCCGCCAAGCAGTGCAAGATAGAGAGCACTAAACAGTTCCACA11.3888888888888888No Hit
AAAAGAACGCTCGAAAAACTCCGATTTCGTCAGCCGAAGAAAAGAGAAAT11.3888888888888888No Hit
GAATACGTGTAATACGAAGTTTCAGCTGGGGATGCATAATCGTCGTAGTA11.3888888888888888No Hit
ATGGTGAAGTTGAGATTCCTGAGAACTGAAGAATTTGGAAATGATGGTGT11.3888888888888888No Hit
AAGCAATAGTAAGAAGACAGATACCATGATAGTAAATTCAGCGAGTGCAA11.3888888888888888No Hit
GTGCGAAGACAGTTCATATAACCATTGCTTAAGGACCACGAACATAAGGA11.3888888888888888No Hit
GTTCACTTCAAACCTACCACCCAAGTCAATGGGAGCTAGAGTCAGAGTCT11.3888888888888888No Hit
ACAGATAAGAGAGAAGGATATATGGGATTATGCTTCTACGATTAACTCCA11.3888888888888888No Hit
CAATGGAGAAATCGAAGCTGAGCGCGGAAAAGATGGAACATGACAAAGCT11.3888888888888888No Hit
CAGAGCATGTCCCTTTTTAAGCACAGTATATGAACAAGCAAGAGCCACTT11.3888888888888888No Hit
ATGGGGATCCCAGAAGATGCCAAGTGCGACATATAAGTGCAAGAGGAGTC11.3888888888888888No Hit
GATCAGAGGAATCCACGGAAATTGTCAAAACGCACACAAGCAACGACAAT11.3888888888888888No Hit
GTTACAGCCTGCGTGATCCGTGTGTATGGGGAGGATGGTTTGTCTCTTGT11.3888888888888888No Hit
GATATTGAGAAGCCTCTGGCGGATCAGTGTGAGGCTAAGAGGGCGAAGAC11.3888888888888888No Hit
ATATCAGCTTAACAAACATGCTGAGCTTGAGAATAGGAAGCAAAAGCTCA11.3888888888888888No Hit
ATACAATTTTTTGTACAATACATACAACAAAGAAACGCAGAGGTTGAATC11.3888888888888888No Hit
CGTGCTATTCAACGAGGACAAGAATACTAGTGAAGAACAAGATTGAGTAC11.3888888888888888No Hit
GTACTAAGCCAAAGCCTAACTTCTCTGACAGAGAGCTCTCCTTTCTTCTC11.3888888888888888No Hit
CAAATTTTCAACTAAGAACAAAACTAAAATTCGAAAGAACAAAGTTAGGA11.3888888888888888No Hit
AGTAGCATTACTCATTCCTCACTTGTGAGACACGTTGGAATCAAATCTTA11.3888888888888888No Hit
GTGTGGTATAAAAAGAACAAGCCAAGGTCTCCCTTGGATACGATTAGTGA11.3888888888888888No Hit
GAAAAAAAGAAGCTGGAGTATACTATTTATAATGATATAAGATTTCAAAA11.3888888888888888No Hit
AGGGAATAAAGGAAAAAGAAAACCGAAGTAATAATAAAATTGAGAAAGAG11.3888888888888888No Hit
ATAGATGTAACAATCCCTCAATAGTAGATAAACCCTACTGTCGCTAATGT11.3888888888888888No Hit
AACTCCAAAAGGTGAGGATGCAAATGAATGCGAGAGGTTCGCCAAGTACT11.3888888888888888No Hit
GGAAGATATGGTGTATGTTGGTTACACTTTGACCACCATAGATGCACAGG11.3888888888888888No Hit
AAGCAGGTGTAGTCCAGAGTATGAACGTGTCGAAGGGTATCAGACCAGGC11.3888888888888888No Hit
TTTACAGAAACGAGGTTCATCACTCTGTAAGTGAACAAACTCAGATCCTA11.3888888888888888No Hit
ATGGAGAAACACGAGTATCACCTTCACAAATACCTTCACCCGGAGTACCA11.3888888888888888No Hit
CATGAAACAATAATCAACATCTCAGATGTGGATACTGTATTGATCTCTCG11.3888888888888888No Hit
GAAGATTAAAGAAAATTGGGTTGTTATGTTGTAAATATATGATTATTAGT11.3888888888888888No Hit
AGGTACCGTGGCGGAGCCACCACACCACCAACATCAGCAGGCACCACACC11.3888888888888888No Hit
TAAGGAAGCTTGCAACCCGGAAAAACTAAGTGGAGGGGCAAAGGATCCAC11.3888888888888888No Hit
TCACAGGAGCATAAGAAGTTAAAGTCAGATGAAGGGGGGGAATCGCGAGT11.3888888888888888No Hit
TGCATGCTCATGGTCATCCCCGCCCAGAATCCATGCCCAGAAGAATATGT11.3888888888888888No Hit
AACCCCAAGGCTGGCTAGGCTCCGTAGCCAAGAAGACGAGTGAAGAAGAA11.3888888888888888No Hit
AAACAGAACACGGAGCAAAATCAGGACACGACAACGCAGATGCCGAAAAC11.3888888888888888No Hit
CACACATTGATTCATAGCATACCTAAAGAACTTATGGTAAAAAATTAGAA11.3888888888888888No Hit
CAGGGGTCTCTCAGACGAGTACATTGGAGCATCGTATTTGAGGGATCGGG11.3888888888888888No Hit
AAAACAATAAAATAAGAAAAAGATAAAAAAAAAATACAATAAAATTCACA11.3888888888888888No Hit
CACCACACCATCTAGACAATCAGTCAAAGACTTTGATTGCTCTTCCATGA11.3888888888888888No Hit
AAGATTTAATCAGAACAATATAGACAACAGATCAACAAACGAGCCACCAA11.3888888888888888No Hit
ATAAATCTAACCGTAACAAAAAAGAAAACTGCAAAATACCAAGAAATCAA11.3888888888888888No Hit
AACTAAATCAAGCAAAAGGGTCAAGTTTACAGATGCTTTCTTCTCAAAAG11.3888888888888888No Hit
GGTACAACAAGAAGAAACTGACTTACGAAGAGAGGAAGAACAAGTTGATC11.3888888888888888No Hit
GTTTTCTTTTCTGAAATCTGATGAGACCCAAAGTCTATGGATATTGTGTT11.3888888888888888No Hit
CAACAACGCTTATGATAGTCATAGAACAAGTCATCTCATATGGTTTAATA11.3888888888888888No Hit
GAAAAGTGACGGGTGCGAATTCAAGAAGGGAGCCGAGGAAAAATACAGTC11.3888888888888888No Hit
AAAAACAGTTATCAAAGCCGACTACATGGCATAAAATATTCCAAGAATGC11.3888888888888888No Hit
CAACCATCCACTTGAATCATATCCCAATAAACTTAGAGTGAAAAATAGTT11.3888888888888888No Hit
TTGAAAAGAACCATCTTACCTAAATTACGGAGACAGTATCTGAAGCAAGT11.3888888888888888No Hit
AAAAAGTCTTATTTTTTTGATGTCATGCTCATGTTATATCTAAACTTGGT11.3888888888888888No Hit
GCTATTTGCTGGTGGTAGGGAGAAGTTATCTTCAGTACCATCAGGAGGTG11.3888888888888888No Hit
GTTGCGTACACTGAATGGTACGGACAATGGTGCATAAAAAAATCTGTTAA11.3888888888888888No Hit
GTAGAAAGCACACGACGAAAAAACATCCCATTAGGAATTAATTGATTATA11.3888888888888888No Hit
CAAGCATGTGACAGCTATGCGAGGCATCCCACACTAAGCTATTATCAAGG11.3888888888888888No Hit
TTCCCGTTTGCACGCAGGTTGTCGTCGGACTCAGCGGGTAATGCTACGTC11.3888888888888888No Hit
GCCGCATATAGTGTATTTAAGAGTTTAGATCACATGGATGATAGTATGAT11.3888888888888888No Hit
CAAACCGTCGCCACTGCAAGATCCCAGACTGGTATCTTAACACACATAAG11.3888888888888888No Hit
CCAGCGCCATCATCACTAATGATGGGATCGGGATCAATCCTCAACAGAGT11.3888888888888888No Hit
AAGAAGTGGAATCAACCCAGAGTACATGGTGTAAGAGAAAACTTCGACCA11.3888888888888888No Hit
TTAACACATACAGGATATATGAAAAAAAGCTGAACAACGTGGCACAAGAC11.3888888888888888No Hit
CTCTCATAAGCTAATGATGTATCATCCTTAGAATATTAAGAATTATATAG11.3888888888888888No Hit
TGAATAAAAACTACATAACTAACAACAAGAGTAGAAAAGATTTTTGTAGT11.3888888888888888No Hit
CAGCAGCGCAGCAATTTTCGGGCTTTTTCTAGGTGCGGGCAGTTTGATTC11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGCT50.085.08
GAAGCTG50.085.09
GAAAAAA50.085.01
AAAAGAA50.085.05
AAGAAGC50.085.07
AAAAAGA50.085.04
AAAAAAG50.085.03
AAAAAAA50.085.02
AAAGAAG50.085.06
Produced by FastQC (version 0.11.5)