FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494828046
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG11512230.23265112179999595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA772500.016.6660791
ACCCCAT887600.016.5765764
CCGGACA855800.016.3732761
CACTAAG1097400.016.1946011
CCGAACG1076150.015.8958111
GGAACCC1287350.015.7905311
AACGCAC940050.015.5861554
GGCACGT1381200.015.2322121
CAACCCA914300.015.1876193
GGGATAG651500.015.0563693
AGCCGAA518250.014.9891858
GGCCCTA736800.014.9709310
TACCGAA833000.014.9457761
GAACCGA747800.014.92281110
TGGGCTG862850.014.9171281
GGTTAAC1227250.014.9150521
AGCCGGA526600.014.87266358
AACGGGA686400.014.7683751
AGGGAAG617900.014.7641793
AGGCTGC1099350.014.6946131
Produced by FastQC (version 0.11.5)