FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n01_Ctr_9h_14h_cutOct25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n01_Ctr_9h_14h_cutOct25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACTCAGTGCTCATTGATCAATTTTT11.2345679012345678No Hit
AGCGCCACATTCGGGCCCGACATCACCT11.2345679012345678No Hit
GTCATTTGTTAAACAGAGTAAGGACGAA11.2345679012345678No Hit
TGATTCTTCTCACCCAGTTCGTCCAATG11.2345679012345678No Hit
GAATCGTAGCAGAAAGGCCTGCAGACCC11.2345679012345678No Hit
TTTCACAAGATAGTCAGAGAACCAAAGT11.2345679012345678No Hit
AGGCTGCGTCAAATCCGTCTTCGATCCA11.2345679012345678No Hit
GACTGATCATGCCGACCTATTGGCGACC11.2345679012345678No Hit
CACAGGCTCTTCCGTGCCCTCCGGCGTT11.2345679012345678No Hit
AGATCCAAGGCCTGAATGTTATCAATTA11.2345679012345678No Hit
GGGTATTCATCAGCGCCTTTACTGTATG11.2345679012345678No Hit
CCTATCGGTAAGTTAGCTACATTTTTGG11.2345679012345678No Hit
GATCATGGTAGTCGGAATAAAAGAAGTT11.2345679012345678No Hit
CATTCATTCTAATTCCTGAAACATCTAC11.2345679012345678No Hit
CTCCGATTCTTTGCGCGGCCACACTGGG11.2345679012345678No Hit
AATGACCGTTCCTTGCCTTCGTGAGGTA11.2345679012345678No Hit
GGGACAAAGACGCCAACTTCCTATCCAG11.2345679012345678No Hit
GATTCTTAGGTTACAATTCACTGGCATT11.2345679012345678No Hit
AGGCCACTCGTAGTGTATACCCTCCACC11.2345679012345678No Hit
CAGATCATCAGGACAGGACAGGTCTGGA11.2345679012345678No Hit
GCGTGCAGTTAGGCCCCTTTTCACCAGG11.2345679012345678No Hit
AGTACTGCATCCTTCGTACTGCTGGTTT11.2345679012345678No Hit
TCTTCCTATGTCCATAAGCGGGATCCGC11.2345679012345678No Hit
GCACGGTTCTAGTGACTTCCTTCATGGA11.2345679012345678No Hit
TTTACTGCAATTCTCTGTCGCCGCCCCA11.2345679012345678No Hit
TGGTGTTGTTTTGTGGCTTGTGTTGTGG11.2345679012345678No Hit
TTGACCCTCGCTAATGGCTGTAGGCATT11.2345679012345678No Hit
TCATACTTCCGAGGCTTCAGTTTACAAA11.2345679012345678No Hit
ATGAGGGGTCTTGAACTCTGAAGCATAC11.2345679012345678No Hit
TGTTCCGCACCAGTTATCTCAGCAGTTT11.2345679012345678No Hit
AAGCGAAAGCCTCAGCGACATTATGCCG11.2345679012345678No Hit
AAAGGGCGTGATTCTGGGTTCATTATGT11.2345679012345678No Hit
GCTCAAAGTCCACACGTACTCTGGTATG11.2345679012345678No Hit
CTATCCGCATGCACTACATCCGTCCGTC11.2345679012345678No Hit
AGGCCACGTACTTCCCTAGGCCTAGGCA11.2345679012345678No Hit
CTACCCAAGGAGGCAGGTTTTGAAGTGC11.2345679012345678No Hit
AACACACCAAGCTGCCACTCTCAGCATT11.2345679012345678No Hit
CCCAACTGTGCTCGTGTGGTATTTATCG11.2345679012345678No Hit
GGTGGGTGGGTTGGTGGGTGGGGGGGGG11.2345679012345678No Hit
CATGAGTAGATCCGAGACGCTCTATTTG11.2345679012345678No Hit
CCACGAGGTGACTCTATGCCACTGGCCT11.2345679012345678No Hit
GTGCTGGCATGGGTTTATGTTAGGAACT11.2345679012345678No Hit
CGGAGAATCGCTTTATGTTCGTAGATCG11.2345679012345678No Hit
CTGCCTATCCATACAGGCCTCGCATTGT11.2345679012345678No Hit
TGAGCTCATTGTGTTTCCATTGACCGTG11.2345679012345678No Hit
GATAGCTAGGAGGCAGTGTATAACTATG11.2345679012345678No Hit
CTACCTGCAGCCCAGTCTACAATGTCGG11.2345679012345678No Hit
GGGGGCGCAGCGTGGCCCGGCCGCGTAG11.2345679012345678No Hit
CGGGACTAGTCGAATAATTAAACTTATC11.2345679012345678No Hit
ATTCACTAGTGTTGTCTGATCGGTTTCT11.2345679012345678No Hit
TATCAGGAGGTGCCAACTTTCGGGTTCA11.2345679012345678No Hit
CATAGACTCGTGTGATCTCGTATTTGGT11.2345679012345678No Hit
TACATTCTCCTCACGTGTGTCATATAGA11.2345679012345678No Hit
CCTACGTGTCCTGGTGTTCGTTATCGAT11.2345679012345678No Hit
ATGACCAGTCTCGACGTTAGAAGACATG11.2345679012345678No Hit
ATCGTAGAGCCATGCCGGTTGGAATAAC11.2345679012345678No Hit
GGAGCAAGTTAATCGCTCAGTGCAGGCC11.2345679012345678No Hit
TAAGCCATCCGAAGGATCCGGTAGAATT11.2345679012345678No Hit
CATGCTCTCAGACATCGGATCCGCTGGT11.2345679012345678No Hit
CTTCCGAAGCCATTCAAGTCATCGGGAC11.2345679012345678No Hit
GAGACCCTCTAATTCCGGATTTTCCAAT11.2345679012345678No Hit
GAACGTTGTGCCCTTTTGATGCGTTGTA11.2345679012345678No Hit
ACAACCATCGACCTAAGTATCCTTCATG11.2345679012345678No Hit
CTCTCGACAGCTGGTCAGGATCAGGAAC11.2345679012345678No Hit
TATACCTTCTCGTTTATGTAACTTATTT11.2345679012345678No Hit
GACGTGCGCGGAGGTAGGCCGTAAGCAC11.2345679012345678No Hit
TTGCTGCCAATTGGTCATTCTCTGTACT11.2345679012345678No Hit
TTTCATGAGATCCGAGTTGCGCATTCGT11.2345679012345678No Hit
GTGTGTTTGTGGGTGTTTGGTGTTTGGT11.2345679012345678No Hit
CGGGTGTCAACGGCCTAGTCTGCACACA11.2345679012345678No Hit
CCGATCTCAACATACCACTCGCACGAGG11.2345679012345678No Hit
TCCGATCAGCCTCTCTCCTCTAACTCAT11.2345679012345678No Hit
CTCAATTAGTGTTGAACTTATAAGCCGT11.2345679012345678No Hit
AGCGTATTCTCAATCTAGTTGCCTGGTG11.2345679012345678No Hit
TCGACGGGTGGATCGAAATTACGTCACT11.2345679012345678No Hit
AATGAAGTCGGTCACGGGGGGTGGTCGG11.2345679012345678No Hit
TTACGCCCAGAGGGTTCTCTAATAGATT11.2345679012345678No Hit
GCCGATGGTTTACCTTCATTCATGCTGC11.2345679012345678No Hit
ACCAAACCAGCACGAAGGTTCCGTAAAA11.2345679012345678No Hit
GCCATGGGTTCCTAGACGACGCCATTAT11.2345679012345678No Hit
TATCTTGCATCCTAAGCCCGGACGTATG11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGA50.022.05
GGTGACT50.022.07
TGACTCT50.022.09
CCACGAG50.022.01
TATGCCA50.022.015
CACGAGG50.022.02
ACTCTAT50.022.011
CACTGGC50.022.020
CTCTATG50.022.012
TCTATGC50.022.013
CGAGGTG50.022.04
AGGTGAC50.022.06
CTGGCCT50.022.022
CCACTGG50.022.019
ACGAGGT50.022.03
TGCCACT50.022.017
ATGCCAC50.022.016
GTGACTC50.022.08
GCCACTG50.022.018
CTATGCC50.022.014
Produced by FastQC (version 0.11.5)