FastQCFastQC Report
Tue 5 Nov 2019
HJ5GNBGXC_n01_9hCut_9h14h_Oct25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5GNBGXC_n01_9hCut_9h14h_Oct25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACACAGACAACATTATTACAAAGAG11.3888888888888888No Hit
ACCCTCCCACGTTAGAGGGCATACGGAC11.3888888888888888No Hit
GGGTCTTCAAGTGGTCAGGATTGTAAGC11.3888888888888888No Hit
ATAGGCTTCACACCGGTTGTTATGTCCA11.3888888888888888No Hit
GGGACTCGATAACTCGCAATATGGACTA11.3888888888888888No Hit
CGCAGAACACGTCAAGCATATGTCTTTG11.3888888888888888No Hit
CTACCCATCACATACATCGTGTACTAGG11.3888888888888888No Hit
ATCCCTAGTCTTCACATCATTGATCCAT11.3888888888888888No Hit
GGGAGCTAGTAGCATCATTTAACGGGAG11.3888888888888888No Hit
GCGGGCAGCACTCCGAGCACAAGTACCG11.3888888888888888No Hit
GAATTTCAGTTCATCGACATCTTAAGTT11.3888888888888888No Hit
CTGGTCATCTTCACGCCCACTGTTGGGT11.3888888888888888No Hit
AAACGCTCATCTTTCAACCCTTAACCCA11.3888888888888888No Hit
TACAACGGTACGAGCAGAAATCCTCCTC11.3888888888888888No Hit
CATCACGCAGGGAAACAGCCTAGCGAGA11.3888888888888888No Hit
ATACGTTCATGAGAATACCAAGTCCTTG11.3888888888888888No Hit
CTGTATTCACGACGCTTGCCTTCTCGCC11.3888888888888888No Hit
AAGTAGACAAAGTCCTCTAAATTGATCG11.3888888888888888No Hit
GACAACAGTCGAGGTGATAACAATGCCT11.3888888888888888No Hit
GCCGAAACATATCCACGCTCGAACTTAG11.3888888888888888No Hit
GTGCGTGAGTTCCGTATCCTTGAAATCT11.3888888888888888No Hit
CTGACTTACACAGCGTTCAAAATTACAA11.3888888888888888No Hit
TACAATTAAAAACTAACAATTATAAATA11.3888888888888888No Hit
GACACGCGTACGATTAGAAATCTATATG11.3888888888888888No Hit
CTCCTTTGTATGCTTGGAAGCTCGCTCA11.3888888888888888No Hit
CAGCAAGTCTCCTTCCTTTCCTTCTAAT11.3888888888888888No Hit
TCAGGCTAGTAATTAATTAGAAAATATG11.3888888888888888No Hit
AAGGTAAAAACAATACCTCTTCAATTTA11.3888888888888888No Hit
GGGTCACCATCGGCCAGCCCCGGGCGGT11.3888888888888888No Hit
GATAGACAAGTTAACTGTGCTAACACTG11.3888888888888888No Hit
AGCGGTGACCTAAACAGCAACGCAATAA11.3888888888888888No Hit
CACTAAACAAAATCATATATGAACTTTA11.3888888888888888No Hit
CATGGTACACCGTACGCGCCCGGTGCTG11.3888888888888888No Hit
GGGGTGGGTGCCGGCTCTGTCGACCCAA11.3888888888888888No Hit
GGGGCGTGTAGTGCGGCATGTTGCGTCG11.3888888888888888No Hit
ACTTTCATCGTTCCTGTGCGTTGTCCCG11.3888888888888888No Hit
GGCCTAACAATTGTGCGAATCTGGGGTG11.3888888888888888No Hit
GGAAGAGCAGACTGCCCGTACCGTTGGA11.3888888888888888No Hit
TAAGTCAGACAGAACTCAATTGGAAGTA11.3888888888888888No Hit
GTTCACAAGCTTACGTATTTATCCATTT11.3888888888888888No Hit
TACTGCCAGCGATCGATGCAATCTCTGT11.3888888888888888No Hit
CGGAGCATCGCGTTATTTCGTCGTTTTG11.3888888888888888No Hit
GACGTTATCAGCTTGATTGCATAATTAT11.3888888888888888No Hit
AAACCAAAGATCAAGAAACAATTTAATG11.3888888888888888No Hit
AAACCTTCAGACAAATATACGGGTTAAG11.3888888888888888No Hit
CATGCTCCAAGAGGATAAGGTAAGAGCC11.3888888888888888No Hit
AGGTTGTAGCCTCACGTATCTTCTCTTC11.3888888888888888No Hit
ACAGTGAGAAAGCTGGCATATATTTTCG11.3888888888888888No Hit
CCATCCGGTTAGTTGGAAGATTTACCAC11.3888888888888888No Hit
GGGACCAAGCGTGATTTCAACAACTAGT11.3888888888888888No Hit
GCGTGCCTCGCACACTCGTTTACGACTG11.3888888888888888No Hit
ATAGACCAGCGATTCTTACCCCTCCCAC11.3888888888888888No Hit
TGGTGGAGTTTCGTTGGGCTTACCCATC11.3888888888888888No Hit
TTCACCGAGGATGCGTACTCTAGCTGCG11.3888888888888888No Hit
CTACGTCTCATCCCTGACCCTCGTTAGG11.3888888888888888No Hit
TAGACTGCACCTACTTCAGAGAGATAAT11.3888888888888888No Hit
AATGACGAGAGCGACGGTATTATTTTAT11.3888888888888888No Hit
TGCGACGGTAAGCGGTAAGACTCTGTAA11.3888888888888888No Hit
GGATCTATCTGCGGCAAAACCCTAAGTT11.3888888888888888No Hit
TCTCCAACAAAGGGCTAGTGTTTTAATC11.3888888888888888No Hit
TAGCACAAGAACGCGTTTATGATCTCTA11.3888888888888888No Hit
TCACACCAGGCCACCTTCTAGCGGGATC11.3888888888888888No Hit
GAGCCTGTTCCAAAGGATAATCTGAAAG11.3888888888888888No Hit
GCGCCCGAGCGTGGTGGGGCTTGGCCGT11.3888888888888888No Hit
TTAGCGAACCTACCAAAATACAAAAATA11.3888888888888888No Hit
AAAGCGCCAAGTCGTTAAACATTATCGT11.3888888888888888No Hit
ACTCAAATCCTTTTCGTATTGAGGGGGT11.3888888888888888No Hit
AGGAGGTAGAGGAGAGTTATTTGTCGAG11.3888888888888888No Hit
CCGGACAAGCACGATGATGTTGATGTCA11.3888888888888888No Hit
AGCTACAAGATTCAAGTGAATTCATAGT11.3888888888888888No Hit
AATCCAAAGCCAAAACAAAATAATTACG11.3888888888888888No Hit
ATATCCTTAGCAGTTAGAAAGTGATAAA11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG50.022.022
ACGTCTC50.022.03
TGACCCT50.022.015
CCTCGTT50.022.019
CATCCCT50.022.09
CCTGACC50.022.013
CTGACCC50.022.014
TACGTCT50.022.02
GTCTCAT50.022.05
ACCCTCG50.022.017
CCCTGAC50.022.012
TCGTTAG50.022.021
CTACGTC50.022.01
GACCCTC50.022.016
CCCTCGT50.022.018
TCATCCC50.022.08
CTCATCC50.022.07
TCTCATC50.022.06
CTCGTTA50.022.020
CGTCTCA50.022.04
Produced by FastQC (version 0.11.5)