FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_456glu2.35200000052731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_456glu2.35200000052731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326022
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG373750.7017432522809707Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG388550.042.43636318
CACACGT323450.042.2632819
AGCACAC399300.042.2515917
GAGCACA432050.042.24045616
AGAGCAC433400.042.17635315
ACACGTC245200.041.5966620
ACACGTA83750.041.15014620
ACGTCAG117750.041.1141222
CACGTAG83950.040.8120921
AAGAGCA476650.040.58048614
CACGTCT131650.040.3616321
CACGTCA120600.040.17982521
ACACGAG67600.040.16594320
ACGTCTG102650.039.77657322
CACACGA69000.039.7407219
CGTCAGA122900.039.4469323
CGTCTGA102500.039.37462223
CACGAGA70800.038.3198421
GTCTGAA95300.037.0380324
CGTCTAG32650.036.51829523
Produced by FastQC (version 0.11.2)