FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_456glu.35200000052717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_456glu.35200000052717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293196
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG314240.5936677954113168Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG317700.042.42384318
AGCACAC327150.042.1473817
CACACGT265400.042.10378619
GAGCACA357500.041.9171116
AGAGCAC358750.041.8149515
ACACGTC201450.041.45577220
ACACGTA68900.040.424620
CACGTAG68700.040.14968521
ACGTCAG97200.040.1357422
CACGTCT110450.040.10775821
ACACGAG56300.039.8810420
CACACGA57000.039.8239419
AAGAGCA401850.039.60718514
CACGTCA99100.039.45704321
ACGTCTG86600.039.3069622
CGTCTGA85900.039.18202223
CGTCAGA100650.038.69293623
CACGAGA59050.037.98600821
GTCTGAA81600.035.87034624
AGATAGA83400.034.8504187
Produced by FastQC (version 0.11.2)