FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_456gal2.35200000052774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_456gal2.35200000052774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6314498
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82150.038.6909118
CACACGT65800.037.67328619
AGCACAC88050.037.17102417
GAGCACA97500.036.98280716
AGAGCAC97900.036.831715
ACACGTC46000.036.82351720
ACGTCAG31700.032.57410422
CACGTCT20400.031.76006521
CACACGA22400.031.53257419
AAGAGCA122300.031.28566214
CACGTAG25200.031.06688921
GATCTCG425400.030.7153345
CACGTCA34150.030.56654421
ACACGTA26000.030.36839920
ACACGAG24100.029.2156420
ATCTCGG132900.029.13592145
CGTCAGA35450.029.12832823
ACGTCTG15600.027.54405222
CGTCTGA16250.026.02696823
AGATCTC916250.023.96100245
Produced by FastQC (version 0.11.2)