FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_456gal.3520000005275b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_456gal.3520000005275b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG253.397964E-545.00000422
GTCTGAA253.397964E-545.00000424
CGTCTGA253.397964E-545.00000423
ACACGTC301.7906041E-644.99999620
CACACGT301.7906041E-644.99999619
CACGTCT301.7906041E-644.99999621
AAGAGCA301.7906041E-644.99999614
GAGCACA301.7906041E-644.99999616
AGAGCAC301.7906041E-644.99999615
AGCACAC301.7906041E-644.99999617
GCACACG301.7906041E-644.99999618
CGGAAGA959.650092E-618.94736911
GGAAGAG959.650092E-618.94736912
GAAGAGC952.2237294E-416.57894913
TCGGAAG850.002149441515.88235310
ATCGGAA850.002149441515.8823539
Produced by FastQC (version 0.11.2)