FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_455glu2.352000000526fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_455glu2.352000000526fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5146289
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG415510.8073973303870031Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG53070.10312285221447921Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG391350.042.50631718
CACACGT336450.042.38236219
AGCACAC402400.042.27823317
GAGCACA436500.042.2426716
AGAGCAC438250.042.13558215
ACACGTC254300.041.91357420
ACACGTA86000.041.32992620
CACGTCT136250.040.96660621
CACGTCA122950.040.93334221
ACGTCAG123600.040.6270722
AAGAGCA479100.040.61742814
CACGTAG87000.040.44429421
CGTCTGA107750.040.1101323
ACGTCTG108300.040.0722322
ACACGAG56850.039.72914520
CGTCAGA126650.039.68459323
CACACGA59800.038.89593519
GTCTGAA101650.037.84747724
ACGTAGA94150.037.56401422
ACGTCTA33750.036.86303722
Produced by FastQC (version 0.11.2)