FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_455glu.352000000526d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_455glu.352000000526d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221815
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG135650.42103596885606404Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT114500.042.0634919
GCACACG135250.041.8140418
AGCACAC139900.041.5176417
AGAGCAC154600.041.3240615
GAGCACA154450.041.18941516
ACACGTC89000.040.99918420
ACGTCAG46800.039.65785622
ACACGTA29350.039.39756420
AAGAGCA174600.039.01146714
CACGTCT45550.038.77058421
CACGTCA48500.038.2677821
ACACGAG21550.037.99867620
CGTCAGA49250.037.68502423
CACACGA21900.037.3896519
CGTCTGA35700.036.9275523
ACGTCTG36650.036.58418322
CACGAGA23050.035.7216321
CACGTAG32650.035.41612221
GTCAGAT54100.034.30660624
GTCTGAA34100.033.9101624
Produced by FastQC (version 0.11.2)