FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_411glu.3520000005269f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_411glu.3520000005269f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458607
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33900.039.41717518
CACACGT28150.038.8379819
ACACGTC22750.037.47670720
AGCACAC36350.037.37930717
GAGCACA40100.036.2398916
AGAGCAC40550.035.83772315
CACGTCT13600.033.74424721
CGTCTGA10700.031.7472823
ACGTCTG11950.029.74388322
ACGTCAG13650.028.6764322
CACGTAG8750.028.28089121
GGTCGCC7500.027.9143444
ACACGTA9700.026.67041220
GTCGCCG8000.026.16969545
AAGAGCA64250.025.94411814
CACGTCA15450.025.77230621
CGGGGTG3800.024.271361
CGGGGAG4300.023.5417061
ACACGAG9800.023.41411220
CGTCAGA17850.022.68546323
Produced by FastQC (version 0.11.2)