FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_411gal.352000000526b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_411gal.352000000526b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146510
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42500.039.7511118
CACACGT39150.036.71701419
AGCACAC47850.036.1528617
ACACGTC30600.034.92054720
GAGCACA52800.034.72337716
AGAGCAC53300.034.43984615
CACGTCT17850.032.39078521
GCGGCGC12350.031.3294471
GCGGGGC5700.029.5990371
CGTCTGA15600.028.84243623
ACGTCTG16450.028.17265922
ACGTCAG16850.028.03792822
CACGTAG13050.027.92743521
GGGGCGC11000.026.3808161
GTCGCCG8450.025.8397945
CGGGGAC7650.025.2888011
AAGAGCA86650.024.6632214
GGTCGCC8700.024.57979644
CGGGGCG3100.023.9465771
CGCTACC25950.023.9256045
Produced by FastQC (version 0.11.2)