FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_410glu.3520000005265c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_410glu.3520000005265c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872905
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG199750.041.77107218
CACACGT176150.041.35266519
ACACGTC135550.041.0269620
AGCACAC207750.040.82274217
GAGCACA222100.040.39322316
CACGTCT79100.040.30217721
AGAGCAC224250.040.1764915
ACGTCAG60550.038.86446822
CGTCTGA65050.038.77012323
ACGTCTG65400.038.4935122
CACGTAG44950.037.78787221
CACGTCA63950.036.72781821
ACACGTA48200.036.21888420
AAGAGCA263650.036.17669714
CGTCAGA65900.035.57305523
CACACGA29850.035.11970519
ACACGAG29250.034.99499520
GTCTGAA66550.034.14382624
CGTAGAT52150.032.5278923
CACGAGA32000.032.1293121
Produced by FastQC (version 0.11.2)