FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_410gal.35200000052675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_410gal.35200000052675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452264
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22450.036.77519218
ACACGTC16800.033.47806520
CACACGT20900.033.2596319
AGCACAC27000.031.32775917
GAGCACA29150.031.0236416
CACGTCT10550.030.92040321
AGAGCAC30000.030.4445815
CGGGGAG5450.029.7191491
GGTCGCC3550.025.9976444
ACGTCAG8900.025.02532222
CGGGGTG4600.024.4518951
CGGGGAC7100.024.3967931
ACGTCTG10700.023.33854322
CACGTAG5700.023.28663821
CGGGGCG2650.022.0712951
CGTCTGA11000.021.88394423
GTCGCCG4450.021.24553945
CGGGGGT5800.020.5564561
AAGAGCA54000.020.45446214
CACACGA6800.019.84940719
Produced by FastQC (version 0.11.2)