FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_409glu.3520000005260b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_409glu.3520000005260b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160142
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG92550.041.538818
CACACGT79350.040.879619
ACACGTC61050.040.4586920
AGCACAC97450.040.25806817
GAGCACA104600.039.87184516
AGAGCAC105600.039.5794815
CACGTCT35350.039.45520821
CACGTAG19850.038.07858721
CGTCTGA30300.037.419423
ACGTCAG28150.037.3998222
ACGTCTG30700.037.37092222
ACACGTA20950.036.72292320
CACGTCA29300.036.08546421
AAGAGCA126150.035.39603814
ACACGAG15300.035.2870220
CACACGA16050.034.478519
CGTCAGA31850.033.1262523
GTCTGAA30300.031.99952724
CACGAGA17300.031.20808821
CGTAGAT25800.029.03577423
Produced by FastQC (version 0.11.2)