FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n02_409gal.35200000052625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n02_409gal.35200000052625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5869930
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG60960.10385132361033265Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG159850.041.65574618
CACACGT140650.041.12048319
ACACGTC110450.040.65559420
AGCACAC167150.040.5094117
GAGCACA180400.039.6165416
AGAGCAC182650.039.33789415
CACGTCT67700.039.07972721
CACGTAG32250.038.64674821
CGTCTGA56600.038.6351623
ACGTCTG57550.038.19285222
ACGTCAG48600.037.03280322
ACACGTA36300.035.07803720
CACGTCA52000.034.82774721
AAGAGCA224050.034.38836314
CACACGA25150.033.72090519
CGTCAGA53600.033.6202223
GTCTGAA61250.033.05772424
ACACGAG24600.032.8310520
CACGAGA26300.030.88049921
GTCGCCG10450.029.94164545
Produced by FastQC (version 0.11.2)