FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_456glu2.35100000052734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_456glu2.35100000052734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326022
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC379240.7120511330970845TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58250.031.24771345
CTCGTAT60250.030.09841543
TCGTATG61600.029.47531944
TCTCGTA67600.026.52633742
ATCTCGT72450.024.75059141
CATCTCG72450.024.75059140
AGTTCAG55100.021.3099381
GTTCAGA94100.019.1839921
GTGACTG14800.018.7300741
GACGTGT300250.017.7818981
TGACTGG16350.017.0635592
GCATCTC118500.016.99301145
GACTGGA16300.016.9778693
GTAGGGA10650.016.2669732
AGCATCT125350.016.11824244
TAGCATC128950.015.73804843
GGTAGCA246700.015.5405445
GGAGTTC19000.014.8020587
CGGTAGC270250.014.19463944
CTGGAGT20300.013.9649755
Produced by FastQC (version 0.11.2)