FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_456glu.3510000005271a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_456glu.3510000005271a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293196
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC316140.5972573091946718TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC53400.029.87244245
TCGTATG55650.028.58380344
CTCGTAT55950.028.35011343
TCTCGTA62500.025.23502542
ATCTCGT67650.023.11440741
GTGACTG24850.022.763541
TGACTGG27700.020.5497472
GACTGGA28550.020.3319633
GGAGTTC33200.017.5520327
CTGGAGT34050.017.246035
GGATCTC105350.017.06388945
GATCTCG93850.016.6376140
GACGTGT263900.016.4478591
ACTGGAG35150.016.3222734
GTGGATC110900.016.23021343
TGGATCT113350.016.03819844
GTTCAGA92250.015.4398531
GAGTGGA215050.015.30631445
TGTAGGG10000.014.423561
TGGAGTT40450.014.4061186
Produced by FastQC (version 0.11.2)