FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_456gal2.35100000052777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_456gal2.35100000052777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6314498
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAAAA441850.029.24353845
CGTGAAA911200.023.71150845
TGAAAAT166000.023.05484245
GACGTGT52250.021.6487981
ACGTGAA1329600.016.25332644
ACGTGTG72950.015.5751432
GCCCCGG5450.014.4489896
CACGTGA1499350.014.40569343
CCCCGGG4700.014.3611537
TTAGATC185250.013.84562610
GTCCGAC3450.013.6951292
TCACGTG1587000.013.6072342
TGCCCCG5550.013.3778685
GAAAATC68000.013.23478845
GTCACGT1653750.013.06072741
CGTGTGC87150.013.0115523
AGTCACG1705800.012.66351640
TAGATCG754550.012.40427111
CCCGGGT5450.012.3848488
CAGTCAC1756350.012.35156539
Produced by FastQC (version 0.11.2)