FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_456gal.3510000005275e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_456gal.3510000005275e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCAG452.8971159E-530.01
ACACGTC705.830815E-419.28571345
GTTCAGA800.001431875916.8751
TCAGACG850.002149441515.8823533
CAGACGT850.002149441515.8823534
TTCAGAC850.002149441515.8823532
AGACGTG900.003149266615.05
GACGTGT900.003149266615.06
ACGTGTG900.003149266615.07
TTCCGAT950.00451540614.21052717
CTCTTCC950.00451540614.21052714
TGCTCTT950.00451540614.21052712
CGTGTGC950.00451540614.2105278
TCCGATC950.00451540614.21052718
TCTTCCG950.00451540614.21052715
GTGTGCT950.00451540614.2105279
CCGATCT950.00451540614.21052719
CTTCCGA950.00451540614.21052716
GTGCTCT950.00451540614.21052711
CACACGT1000.006349686513.50000144
Produced by FastQC (version 0.11.2)