FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_455glu2.351000000526ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_455glu2.351000000526ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5146289
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC424800.8254491731809076TruSeq Adapter, Index 22 (98% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGA52950.10288967448194224TruSeq Adapter, Index 22 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63550.032.6070845
CTCGTAT63600.032.2984343
TCGTATG66450.031.21790344
TCTCGTA68050.029.52508242
ATCTCGT78850.025.4525341
GTGACTG22650.023.5782151
TGACTGG25350.020.7685282
AGTTCAG72000.020.7496991
GACTGGA26000.020.335853
GGAGTTC29200.018.8007327
GATCTCG110300.018.2360140
CTGGAGT30100.018.014345
GTTCAGA121900.017.912281
GTAGGGA9700.017.3963172
ACTGGAG30900.017.3295124
ACGATCT116550.017.1615838
TACGATC118300.016.9267337
CGTACGA247650.016.61663845
GACGTGT336250.016.5123621
TGTAGGG10200.016.3479121
Produced by FastQC (version 0.11.2)