FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_455glu.351000000526d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_455glu.351000000526d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221815
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC141040.43776566935097144TruSeq Adapter, Index 21 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24100.029.68772343
CGTATGC24200.029.65801645
TCGTATG24900.028.91461644
TCTCGTA27500.025.93542542
ATCTCGT30800.023.0105341
CCGGACA6750.018.362591
GACGTGT111400.017.984221
CCGCCCA17050.017.0506291
GATCTCG43150.016.63323240
GTTTCGA95600.015.90946545
CCGCACA7000.015.7751361
TCGATCT46600.015.20867738
CGTTTCG102250.014.80875544
CCGCACC18450.014.5353561
TTCGATC49500.014.49947537
ACGTGTG138850.014.4377192
CCGCGCA14650.014.3060721
GTTCAGA37500.014.002311
CCGGACG6050.013.7822911
TTCAGAC51750.013.45622351
Produced by FastQC (version 0.11.2)