FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_411glu.35100000052692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_411glu.35100000052692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458607
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG10300.020.3147440
CTCGTAT13250.015.79183643
GACGTGT29950.014.825191
TGCCCCG7000.014.1423045
GTGCCCC7200.014.061954
ATCTCGT14700.013.92802641
CCCCGGG7400.013.3778557
CCCGGGT7750.013.0640058
ACGTGTG36150.012.2609312
GCCCCGG8950.011.31240756
CGTGTGC40900.010.9470043
TCTCGTA21650.010.91181442
CCGGGTA9700.010.9016359
AGTTCAG15750.010.4465561
GTCACAG35000.010.413877540
AGTCACA36150.010.14483239
GTGTGCT44400.010.1347384
CAGTCAC36950.010.10785938
GTGACTG6500.010.0557751
CCAGTCA38950.09.81989837
Produced by FastQC (version 0.11.2)