FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_411gal.351000000526bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_411gal.351000000526bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146510
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG8100.016.1104155
CCCCGGG8250.015.8174987
CCCGGGT8550.015.2624988
GACGTGT36350.013.7653311
GCCCCGG9900.013.6357766
GTGCCCC10250.012.9506594
CCGGGTA12200.012.5404429
CTCGTAT18300.011.43393343
ATCTCGT18150.011.15654241
TAACTAG16200.011.11063130
ACGTGTG45350.010.9642452
AATCTCG19200.010.66360240
CGGGTAA16000.010.54641910
CACATGT46800.010.48031742
TCACATG47050.010.42462941
GTCACAT48050.010.34814840
CAATCTC29650.010.24407745
GTAACCC13350.010.11192213
TCAGACG22400.010.04420953
CAGACGT23000.09.8800084
Produced by FastQC (version 0.11.2)