FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_410glu.3510000005265f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_410glu.3510000005265f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872905
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGA20650.022.5536693
GTGACTG20800.022.53431
TGACTGG22350.020.9388452
GGAGTTC23100.020.7460087
TGCCCCG11050.018.7323575
CCCCGGG11250.017.9993537
GTGCCCC12350.017.307074
CCCGGGT12000.017.249388
CAGATCA45950.017.23550645
AGTTCAG52550.017.066891
CTGGAGT28250.016.5657755
GTTCAGA80150.015.856951
GAGTTCA30600.015.5141498
ACTGGAG31900.014.74084954
CCGGGTA14800.014.59406959
GCCCCGG14450.014.1690426
GACGTGT184500.014.1679061
CATCTCG13850.013.64571940
GTAACCC15600.013.55720413
CTCGTAT15550.013.1667343
Produced by FastQC (version 0.11.2)