FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_410gal.35100000052678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_410gal.35100000052678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452264
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACA22300.015.43656945
CGGCTAC23500.014.64831844
ACGGCTA26750.012.86861643
CCCCGGG5450.012.7976267
CACGGCT26950.012.68963142
TCACGGC27550.012.49493641
CATCTCG7950.012.45230540
TGCCCCG5650.012.3446115
GTCACGG28200.012.20693240
GTGCCCC5800.012.0253544
TCAGACG16900.011.5823523
AGTCACG29950.011.49367239
CGGTCTC5300.011.46178136
CCCGGGT6700.011.4174298
AGACGTG17800.010.9967275
CAGACGT17600.010.99385454
GGGGAGC1450.006831901610.861617
GACGTGT23250.010.5681
ACCCGCG5650.010.3535457
ACATCTC17250.010.30391345
Produced by FastQC (version 0.11.2)