FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_409glu.3510000005260e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_409glu.3510000005260e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160142
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC33930.1073685929303177TruSeq Adapter, Index 5 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG16000.027.6077751
GACTGGA16900.025.827443
TGACTGG17300.025.100222
GGAGTTC20150.021.99677
CTGGAGT21050.020.7355695
ACTGGAG22300.019.4723684
CTCGTAT9600.018.74930243
TGCCCCG5500.017.5902545
GAGTTCA25500.017.2052428
CCCCGGG5700.016.578337
ACAGTGA35700.016.38594245
TGGAGTT27300.016.3180736
GTGCCCC6300.015.356574
CCCGGGT6550.015.113948
CCGGGTA7850.014.3306769
CACAGTG41100.014.01407644
GACGTGT97850.013.3586471
CGGGTAA9100.013.10390710
TCTCGTA14100.013.08461842
AGTTCAG37000.012.7303361
Produced by FastQC (version 0.11.2)