FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l02n01_409gal.35100000052628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l02n01_409gal.35100000052628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5869930
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC64360.10964355622639452TruSeq Adapter, Index 6 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG27350.027.3593271
GACTGGA30150.025.1482773
TGACTGG30900.024.3922562
GGAGTTC33450.022.8018057
CTGGAGT38600.019.8178845
ACTGGAG42400.017.7764344
GAGTTCA43200.017.6555658
TATCTCG16450.016.27596540
GCCAATA62300.015.78189145
TGGAGTT49250.015.5780756
GTGCCCC13150.015.2275464
CGCCAAT67250.014.55333844
AGTTCAG67350.014.5236861
TGCCCCG13750.014.3994425
CTCGTAT18450.014.26773843
CCCCGGG14250.014.0520877
AGCGTCG9200.013.6951216
CCCGGGT14650.013.6684118
CACGCCA66850.013.56342342
GTTCAGA92750.013.5109411
Produced by FastQC (version 0.11.2)