FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t3r-r.35200000052668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t3r-r.35200000052668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12815767
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG157960.12325442558373603Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG278250.041.69349318
CACACGT237750.041.3397519
AGCACAC289850.041.0180117
GAGCACA318300.040.51095216
AGAGCAC321750.040.29331215
ACACGTC182500.040.14743820
ACGTCAG90350.038.24512522
CACGTCT101950.037.51180621
CACGTAG65400.036.80546621
ACACGTA69450.035.50105320
ACGTCTG82900.035.06073822
AAGAGCA407800.034.98404714
CGTCTGA81900.034.88575423
CACGTCA99300.034.86548221
CACACGA50100.034.5747719
ACACGAG51700.032.98234620
CGTCAGA108700.031.95553223
GGTCGCC25900.031.46384244
CACGAGA54250.031.34933721
GTCGCCG27600.029.7705445
Produced by FastQC (version 0.11.2)