FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t2wt.35200000052682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t2wt.35200000052682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14612811
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG319110.21837687492160135Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG676000.041.6602918
AGCACAC696900.041.31781817
CACACGT563350.041.17388519
GAGCACA741950.041.1103216
AGAGCAC745700.041.0363215
ACACGTC432750.040.6401220
ACGTCAG214500.039.9791822
CACGTAG143300.038.9472621
ACACGAG123400.038.70180520
CACGTCT230200.038.6587121
CACACGA125250.038.54351419
CACGTCA223400.038.51630821
ACACGTA146150.038.46483220
AAGAGCA850200.037.7590214
ACGTCTG185350.037.12745322
CGTCTGA183000.037.11377723
CGTCAGA234750.036.54133223
CACGAGA131100.036.51449621
GGTCGCC48750.034.44916544
GTCTGAA181150.033.29588324
Produced by FastQC (version 0.11.2)