FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t2r-r.3520000005264f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t2r-r.3520000005264f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11158563
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG129250.040.67469418
CACACGT113450.039.32007619
AGCACAC138050.038.92906617
ACACGTC88100.038.4800320
GAGCACA151750.037.38613516
AGAGCAC152850.037.20538715
CACGTCT57250.035.7965321
ACGTCTG50350.033.4202722
CGTCTGA49250.032.84471523
CACGTAG30450.032.80150221
ACGTCAG41550.030.75283222
CACGTCA43250.029.7514421
ACACGTA34800.029.28309820
CACACGA26300.028.99613419
ACACGAG25450.028.63883420
AAGAGCA233650.028.30480414
GGTCGCC15150.025.70881344
GTCTGAA59350.024.90527724
CACGAGA29850.024.79417421
CGGGGAC9450.024.7553181
Produced by FastQC (version 0.11.2)