FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t2p.352000000526ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t2p.352000000526ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14324457
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG227550.15885418902789822Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG550400.041.74184418
AGCACAC567200.041.35761617
CACACGT465800.041.24346519
GAGCACA610350.041.02474616
AGAGCAC614150.040.8991115
ACACGTC352500.040.75379620
ACGTCAG170550.039.71566822
CACGTCT195150.038.25929621
CACGTCA178750.038.0317821
CACGTAG126000.037.97477721
ACACGTA128600.037.67931420
CACACGA97450.037.39640819
ACACGAG96100.037.28985220
AAGAGCA711850.037.1652714
CGTCTGA157800.036.72483423
ACGTCTG159700.036.63854622
CGTCAGA189000.035.80445523
CACGAGA101700.035.23652621
GTCTGAA159050.032.40582324
CGTAGAT153050.031.25006523
Produced by FastQC (version 0.11.2)