FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t1r-r.35200000052632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t1r-r.35200000052632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12613810
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCGCTACCATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCC130000.10306164434060765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG143250.040.51498818
CACACGT124300.039.43453619
AGCACAC153750.038.66956317
ACACGTC99750.037.81891620
GAGCACA170550.037.01034516
AGAGCAC174250.036.52139715
CACGTCT62650.035.8342621
CGTCTGA52950.033.94748723
GCGGCGC36500.033.648951
ACGTCTG55100.033.3963722
ACGTCAG45550.033.08902422
CACGTAG32150.032.2558821
ACACGTA34950.030.63717520
CACGTCA53450.028.49245621
CACACGA30000.028.19403619
ACACGAG30000.027.14415220
GTCGCCG19500.027.0159245
AAGAGCA274750.026.78875214
GCGGGGC18500.026.020391
CGCTACC72050.025.8504495
Produced by FastQC (version 0.11.2)