FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_t0r-r.35200000052618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_t0r-r.35200000052618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14049066
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG166870.11877657916903514Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG213100.040.61031318
CACACGT184250.039.70458619
AGCACAC224600.039.211517
ACACGTC143150.038.72062320
GAGCACA245800.038.3005516
AGAGCAC251250.037.71135715
CACGTCT87550.035.278421
CACGTAG48450.034.91552421
ACGTCAG71750.033.6106322
CGTCTGA72100.033.57418423
ACGTCTG73200.033.4672922
CACGTCA74000.032.77038621
ACACGTA52150.032.7401320
CACACGA42050.030.38644819
GGTCGCC29700.030.31987644
AAGAGCA349000.030.29396614
GTCGCCG30050.030.19148445
ACACGAG42550.028.86596320
CGTCAGA85050.028.35626423
GTCTGAA84500.025.87954124
Produced by FastQC (version 0.11.2)