FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_p39550.352000000526c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_p39550.352000000526c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9874023
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG83950.038.64189518
AGCACAC90850.036.62290217
CACACGT73250.036.5778419
ACACGTC59350.035.1373120
GAGCACA100850.035.1329116
AGAGCAC101350.035.09276615
CACGTCT36450.030.55041721
ACGTCAG31300.030.18722722
CACGTAG19350.029.87869621
ACACGAG18150.028.38365620
CACACGA18950.028.37282419
CGTCTGA32350.027.12200523
ACGTCTG34500.026.60476322
GCGGCGC17850.026.339041
ACACGTA23700.025.15399720
AAGAGCA168950.024.45985414
CACGTCA40100.024.01092321
CACGAGA22000.022.9052421
GCGCTAC33300.022.0224324
CGCTACC33700.021.828015
Produced by FastQC (version 0.11.2)