FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_p39532.352000000526ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_p39532.352000000526ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538797
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG332360.31536806335675693Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG317050.041.18218
AGCACAC331300.040.65289317
CACACGT263250.040.49730319
GAGCACA365150.040.26038716
AGAGCAC368900.039.98528715
ACACGTC193100.039.79598220
ACGTCAG109100.038.22952722
CACGTAG77700.037.69585821
CACGTCT93100.037.3804321
ACACGTA79050.037.2797620
CACACGA63050.036.96429419
CACGTCA115300.036.3875821
ACACGAG63850.036.21895220
AAGAGCA449250.035.38689414
CGTCTGA69900.034.4382623
ACGTCTG72500.033.82224722
GTCGCCG52200.033.07647345
CACGAGA70150.033.03035421
GGTCGCC52900.032.55367744
CGTCAGA128500.032.5469923
Produced by FastQC (version 0.11.2)