FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_p39523.3520000005270a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_p39523.3520000005270a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12632426
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG692730.5483744769215351Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG636550.042.04683318
AGCACAC658500.041.68311717
CACACGT532600.041.6412319
GAGCACA718800.041.63192716
AGAGCAC724450.041.47180615
ACACGTC400050.040.90854320
ACGTCAG212900.040.28979522
CACGTAG145100.039.4097321
ACACGTA146950.039.28098720
CACGTCT196150.039.1764321
ACACGAG109850.038.97032520
CACACGA112750.038.96525619
CACGTCA222200.038.7039721
AAGAGCA852250.038.49601714
CGTCTGA154450.037.4487223
ACGTCTG156600.037.39265422
CGTCAGA234900.036.62331823
CACGAGA118950.035.97007821
GGTCGCC100900.034.51660544
GTCTGAA148600.034.09423424
Produced by FastQC (version 0.11.2)