FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n02_p39514.35200000052724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n02_p39514.35200000052724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10674332
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG945110.8854043512980485Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG659150.042.4898218
AGCACAC683200.042.2642817
CACACGT550950.042.19043419
GAGCACA770900.042.11629516
AGAGCAC773700.042.0656415
ACACGTC391050.041.63782520
CACGTAG165950.041.26379421
ACGTCAG216950.041.01066222
ACACGTA168800.040.84696620
AAGAGCA895650.039.89683514
CACGTCT184200.039.8012321
CACACGA116950.039.77865219
CACGTCA225750.039.53085721
ACACGAG117450.039.0157320
ACGTCTG136950.038.60252822
CGTCTGA136100.038.46452723
CGTCAGA233950.038.0605323
CACGAGA123600.037.1472221
GTCGCCG122200.036.80811345
GGTCGCC123500.036.49357244
Produced by FastQC (version 0.11.2)